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GlucoZyme

Direct Electron Transfer (DET) enzyme designed for continuous glucose monitoring, enabling higher accuracy and more robust devices.

Glucozyme Enzyme Structure
Challenges

Why Enzyme Quality Matters

The performance of biosensors strongly depends on the choice of enzyme. Traditional biosensors rely on detection mechanisms based on indirect transduction, which introduce significant operational vulnerabilities.

High Working Potential

Traditional 1st and 2nd generation sensors require high working potentials (up to +700 mV vs. Ag|AgCl) to generate a signal, which increases background noise and opens a broad window of interference.

Vulnerability to Interferents

High working potentials open a broad window where physiological and pharmaceutical substances (such as Ascorbic acid, Uric acid, Dopamine, Acetaminophen, and Salicylic acid) readily oxidize and alter the signal.

Oxygen Dependency

Sensors applying traditional Glucose Oxidase, even when connected with a mediator, is interfered by dissolved oxygen. They suffer from reduced performance and measurement drift when oxygen levels vary (e.g., during exercise).

DET Platform

Direct Electron Transfer Technology

The next-generation proprietary enzyme technology comes with an optimized active site, that allows direct electron transfer to the electrode.

Step 01

Recognition

The proprietary enzyme selectively reacts with the target analyte in sample.

Step 02

Transduction

Electrons released during oxidation travel directly from the enzyme's active site to the electrode, converting bio-recognition into a clean electrical signal.

Step 03

Processing

The electrical signal is amplified and processed to generate an accurate, real-time concentration output.

Comparison

Technology Advantage

3rd Generation (DET)

Direct Electron Transfer

  • No mediator required (direct electron transfer)
  • Completely oxygen independent (signal in absence of O2)
  • Ultra-low working potential ( -100 mV vs. Ag|AgCl)
  • Immune to typical interferents (e.g. Acetaminophen)
  • Next-generation biosensor standard developed by DirectSens
1st & 2nd Generation

Traditional Sensing

  • Mediators required (leaching and deactivation risk)
  • Prone to oxygen concentration fluctuations
  • High working potential required (up to +700 mV vs. Ag/AgCl)
  • Broad window of electro-active interferences
  • No enzyme innovation since the 1970s
Value Proposition

Key Benefits

Applications

Application Areas

Single Use

Disposable blood glucose test strips and single-use reader systems enabling rapid, factory-calibrated one-time measurements.

Wearables

Continuous Glucose Monitoring (CGM) patches and skin-worn wellness devices requiring long-term sensor stability and biocompatibility.

Point of Care

Bedside, benchtop, or clinical diagnostic devices facilitating immediate, high-accuracy bioanalytical results.

Ready to evaluate DET technology?

Contact our team to order enzyme samples or discuss biosensor integration.

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