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GlucoZyme

Direct Electron Transfer (DET) biosensor technology for glucose monitoring, translating into higher accuracy and more robust devices.

Glucozyme Enzyme Structure
Challenges

Why enzyme quality matters

The performance of biosensors strongly depends on enzyme quality. Traditional biosensors rely on older detection mechanisms that introduce significant operational vulnerabilities.

High Working Potential

Traditional 1st and 2nd generation sensors require high working potentials (+100 mV to +700 mV vs. Ag/AgCl) to generate a signal, which increases background noise and degrades sensor accuracy over time.

Vulnerability to Interferents

High working potentials open a broad window where physiological and pharmaceutical substances (such as Ascorbic acid, Uric acid, Dopamine, Acetaminophen, and Salicylic acid) readily oxidize and alter the signal.

Oxygen Dependency

Older generation enzymes rely on dissolved oxygen as an electron acceptor. They suffer from reduced performance and measurement drift when oxygen levels vary (e.g., during exercise).

DET Platform

Direct Electron Transfer Technology

DirectSens develops next-generation bioanalytical systems based on proprietary enzyme technology. By removing mediators, we allow direct electron travel from the active site to the electrode.

Step 01

Recognition

The proprietary enzyme selectively detects and binds the target analyte in the sample.

Step 02

Transduction

Electrons released during oxidation travel directly from the enzyme's active site to the electrode, converting bio-recognition into a clean electrical signal.

Step 03

Processing

The electrical signal is amplified and processed to generate an accurate, real-time concentration output.

Comparison

Technology Advantage

3rd Generation (DET)

Direct Electron Transfer

  • No mediator required (direct electron travel)
  • Completely oxygen independent (stable in absence of O2)
  • Ultra-low working potential (-300 mV to -100 mV vs. Ag/AgCl)
  • Immune to typical interferents (e.g. Acetaminophen)
Next-generation biosensor standard developed by DirectSens
1st & 2nd Generation

Traditional Sensing

  • Mediators required (leaching and deactivation risk)
  • Prone to oxygen concentration fluctuations
  • High working potential required (+100 mV to +700 mV vs. Ag/AgCl)
  • Broad window of electro-active interferences
Industry standard since the 1970s
Value Proposition

Key Benefits

Applications

Application Areas

Single Use

Disposable blood glucose test strips and single-use reader systems enabling rapid, factory-calibrated one-time measurements.

Wearables

Continuous Glucose Monitoring (CGM) patches and skin-worn wellness devices requiring long-term sensor stability and biocompatibility.

Point of Care

Bedside, benchtop, or clinical diagnostic devices facilitating immediate, high-accuracy bioanalytical results.

Ready to evaluate DET technology?

Contact our team to order enzyme samples or discuss biosensor integration.

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